Gene expression of growth factors with angiogenic potential in human dental pulp tissue from teeth with complete and incomplete root development

Abstract

Aim To quantify the expression of angiogenic growth factors (ANG2, VEGFA, TGFß1) and their corresponding receptors (VEGFR1, VGFR2, NRP1 and TGFßR1) in human dental pulps from extracted third molars with complete and incomplete root development.

Methodology Fifty-six dental pulp samples obtained from freshly extracted human third molars were divided equally into two groups according to their stage of root development; 28 third molars with complete root development and 28 third molars with incomplete root development. All samples were processed and total RNA was extracted, cDNA was then synthetized for each sample and the target genes expression profiles for ANG2, VEGFA, VEGFR1, VEGFR2, NRP1, TGFß1 and TGFßR1 were obtained by RT2-PCR. The data was analysed with a Student's t-test to compare the replicate ∆∆Ct values for each gene.

Results Teeth with incomplete root development were associated with a significantly greater gene expression of TGFβR1 (P = 0.03), whereas in teeth with complete root development the genes that had significantly greater expression were VEGFA (P = 0.04).

Conclusion The angiogenic growth factors (ANG2, VEGFA, TGFβ1) and their receptors (NRP1, VEGFR1, VEGFR2 and TGFßR1) were expressed in pulps of teeth with complete and incomplete root development measured by RT2-PCR, with TGFBR1 genes being significantly different in teeth with incomplete root development and VEGFA genes in teeth with complete root development.

Apuntar Cuantificar la expresión de factores de crecimiento angiogénicos (ANG2, VEGFA, TGFß1) y sus correspondientes receptores (VEGFR1, VGFR2, NRP1 y TGFßR1) en pulpas dentales humanas de terceros molares extraídos con desarrollo radicular completo e incompleto.

Metodología Cincuenta y seis muestras de pulpa dental obtenidas de terceros molares humanos recién extraídos se dividieron por igual en dos grupos según su etapa de desarrollo radicular; 28 terceros molares con desarrollo radicular completo y 28 terceros molares con desarrollo radicular incompleto. Todas las muestras se procesaron y se extrajo el ARN total, luego se sintetizó el ADNc para cada muestra y se obtuvieron los perfiles de expresión de los genes diana para ANG2, VEGFA, VEGFR1, VEGFR2, NRP1, TGFß1 y TGFßR1 mediante RT2-PCR. Los datos se analizaron con una prueba t de Student para comparar los valores de ∆∆Ct replicados para cada gen.

Resultados Los dientes con desarrollo radicular incompleto se asociaron con una expresión génica significativamente mayor de TGFβR1 (P = 0,03), mientras que en dientes con desarrollo radicular completo los genes que tuvieron una expresión significativamente mayor fueron VEGFA (P = 0,04).

Conclusión Los factores de crecimiento angiogénico (ANG2, VEGFA, TGFβ1) y sus receptores (NRP1, VEGFR1, VEGFR2 y TGFßR1) se expresaron en pulpas de dientes con desarrollo radicular completo e incompleto medido por RT2-PCR, siendo los genes TGFBR1 significativamente diferentes en dientes con desarrollo radicular incompleto y genes VEGFA en dientes con desarrollo radicular completo.