Modulation of Adipogenesis by EID1 Protein

Abstract

The use of appropriate models for the study of adipogenesis and molecules that can modulate this process are of great importance due to increasing prevalence of obesity (1). Obesity is characterized by excess body fat, which leads to diseases such as hypertension, dyslipidemia, diabetes mellitus and cardiovascular risk (2). Effective therapeutic alternatives have become a strong research center today. We use pre- adipocytes cells 3T3-L1 and mesenchymal cells isolated from abdominal fat of women, to study the adipogenesis process of human pre-adipocytes cells and the phenotypic effect of EID1 when over-expressed in these two models. We found that EID1 decreases lipid accumulation in cells pre-adipocytes in both human and mouse models cells.ObjetiveEvaluate the phenotypic action of EID1 in differentiation of pre-adipocytes cells from human and mouseExperimental procedures3T3-L1 preadipocytes were grown in DMEM medium that contained 10% fetal bovine serum and human preadipocytes were obtained from adipose tissue samples from women with BMI lass than 30 undergoing plastic surgery in the abdominal region. The samples were digested with 200U/mL of collagenase and centrifuged to 200g. The pellet were resuspenden in grow medium (3). To examine the overexpression effect of EID1, cells were grown to 90% of confluence and 1.6ug of EID1 in 4uL of lipofectamina were transfected. 48 hours after the transfection, was induced differentiation (3). Six days after treatment, cells were stained with oil red O and triglycerides levels quantified.ResultsThe over-expression of EID1 decreased triglycerides in 3T3-L1 and human pre-adipocytes induced to differentiation. The phenotypic change were quantificate by removing the triglyceride oil red stained with isopropanol and then measure the absorbance at 510nm The results show significant difference ±SE* p<0.001 for mouse and p<0.05 for human when EID1 is overexpressed. DiscussionIn this study, we describe to EID1 as a modulator of differentiation of pre-adipocytes. EID1 expression is decreased during adipogenesis of 3T3-L1 (4) cells indicating a role of this protein in regulating the early stages of differentiation. We hypothesize that EID1 activated proteins involving in caloric expenditure thereby reducing the accumulation of triglycerides by the adipocyte.