Obtención de extractos con actividad citotoxica in vitro a partir de actinobacterias aisladas de sedimentos del río Guaviare

Ramírez Rodríguez, Laura Cristina

dc.contributor.advisor	Díaz Barrera, Luis Eduardo
dc.contributor.author	Ramírez Rodríguez, Laura Cristina
dc.date.accessioned	2017-04-04T23:10:23Z
dc.date.available	2017-04-04T23:10:23Z
dc.date.created	2017
dc.date.issued	2017-04-04
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dc.identifier.uri	http://hdl.handle.net/10818/30085
dc.description	62 Páginas.	es_CO
dc.description.abstract	Las Actinobacterias son bacterias Gram positivas, filamentosas, de los 22500 compuestos bioactivos producidos por microbios el 45% son de Actinobacterias. Muchos de estos compuestos se han usado para el tratamiento de algunas enfermedades, como agentes antiinflamatorios, antituberculosos, antioxidantes y antineoplásicos, tales como: daunorrubicina, dactinomicina y mitomicina. El propósito del presente estudio fue evaluar la actividad citotóxica de extractos de Actinobacterias de los sedimentos de la ribera del río Guaviare 2°36'19.2"N 72°42'26.2"W (Colombia), para lo cual se escogieron 25 aislados que presentaron previa actividad antiquórum sensing (C. violaceum ATCC® 31532), antibacterial (E. coli ATCC® 23724, S. aureus ATCC® 25923, A. baumannii ATCC® 19606 y B. subtilis ATCC® 21556) y/o antifúngica (T. harzianum ATCC® 52443, Fusarium sp y C. gloeosporioides (aislados de desechos agrícolas). Los aislados fueron cultivados en ISP2 por siete días a 30ºC y 200 rpm y sometidas a procesos de extracción con acetato de etilo. Los extractos fueron preparados en soluciones stock (1mg/mL) disolviéndolos en medio DMEM y posteriormente empleados en ensayos de citotoxicidad mediante la técnica de MTT. Las líneas celulares estudiadas fueron: PC3 ATCC® CRL-7934 (cáncer de próstata), MDA ATCC® HTB-24 (cáncer de seno) y A549 ATCC® CCL-185 (cáncer de pulmón) y como control de línea no transformada se empleó la línea celular L-929 ATCC® CRL-6364 (fibroblastos de ratón).	es_CO
dc.language.iso	spa	es_CO
dc.publisher	Universidad de La Sabana	es_CO
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.source	Universidad de La Sabana
dc.source	Intellectum Repositorio Universidad de La Sabana
dc.subject	Bacterias
dc.subject	Agentes antibacteriales
dc.subject	Extracción (Química)
dc.subject	Compuestos bioactivos
dc.subject	Química farmacéutica
dc.title	Obtención de extractos con actividad citotoxica in vitro a partir de actinobacterias aisladas de sedimentos del río Guaviare	es_CO
dc.type	bachelorThesis	es_CO
dc.publisher.program	Ingeniería Química
dc.publisher.department	Facultad de Ingeniería
dc.identifier.local	264049
dc.identifier.local	TE09020
dc.type.local	Tesis de especialización
dc.type.hasVersion	publishedVersion
dc.rights.accessRights	restrictedAccess
dc.creator.degree	Ingeniero Químico