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Evaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviare

dc.contributor.advisorDíaz Barrera, Luis Eduardo
dc.contributor.advisorSuarez, Zulma Rocio
dc.contributor.authorPastrana Camacho, Natalia
dc.date.accessioned2015-08-05T22:19:57Z
dc.date.available2015-08-05T22:19:57Z
dc.date.available2016-03-03
dc.date.created2015-08-04
dc.date.created2015
dc.date.issued2015-08-05
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dc.identifier.urihttp://hdl.handle.net/10818/17433
dc.description72 páginases_CO
dc.description.abstract​Las actinobacte​rias son bacterias Gram positivas, conocidas por ser las responsables de la producción de la mayoría de metabolitos secundarios bioactivos, especialmente aquellas pertenecientes al género Streptomyces, del cual se han obtenido la mayoría de antibióticos comerciales. Dado las recientes investigaciones en el medio acuático que demuestran el potencial bioactivo por parte de estos microorganismos, el presente trabajo se enfocó en la evaluación de la actividad antibacterial y antifúngica de actinobacterias aisladas de agua y sedimentos provenientes del río Guaviare. Las metodologías de tratamiento térmico, enriquecimiento con carbonato de calcio y siembra directa sobre medios de cultivo selectivos fueron utilizadas para el aislamiento de las cepas, donde el enriquecimiento con carbonato de calcio resultó ser el mejor método de aislamiento de actinobacterias, en contraste al tratamiento térmico. Se aislaron 374 bacterias con características morfológicas de actinobacterias en su mayoría provenientes de muestras de sedimentos, a las cuales se les realizó tinción de Gram para corroborar su carácter Gram positivo, pureza y morfología. Una vez purificados, todos los aislamientos fueron conservados en glicerol a -70 ºC y sometidos a pruebas de enfrentamiento directo contra bacterias Gram positivas, Gram negativas y hongos. En total, de los 374 aislamientos 44 cepas inhibieron el crecimiento de B. subtilis, 36 de S. aureus, 15 de E. coli, 10 de A. baumannii y 2 de K. pneumoniae, además 257 presentaron inhibición de quorum sensing al ser evaluadas contra C. violaceum. En cuanto a la actividad antifúngica 76 de los aislamientos inhibieron el crecimiento de T. harzianum, 91 de Fusarium sp. y 150 de C. gloeosporioides. Con base a los resultados iniciales de actividad antimicrobiana, fueron seleccionadas las cepas que presentaron mayor potencial antimicrobiano. Posteriormente, las 7 cepaes_CO
dc.language.isospaes_CO
dc.publisherUniversidad de La Sabanaes_CO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceUniversidad de La Sabanaes_CO
dc.sourceIntellectum Repositorio Universidad de La Sabanaes_CO
dc.subjectCrecimiento bacterial -- Guaviare, Colombia
dc.subjectResistencia a medicamentos en microorganismos -- Guaviare, Colombia
dc.subjectBacterias patógenas -- Guaviare, Colombia
dc.titleEvaluación de la actividad antibacterial y antifúngica de actinobacterias cultivables aisladas de las riberas del río Guaviarees_CO
dc.typebachelor thesises_CO
dc.identifier.local261047
dc.identifier.localTE07656
dc.type.hasVersionpublishedVersiones_CO
dc.rights.accessRightsopenAccesses_CO
thesis.degree.disciplineEscuela Internacional de Ciencias Económicas y Administrativases_CO
thesis.degree.levelEspecialización en Gerencia de Producción y Operacioneses_CO
thesis.degree.nameEspecialista en Gerencia de Producción y Operacioneses_CO


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