Bioprospecting for culturable actinobacteria with antimicrobial properties isolated from rivers in Colombian Orinoquia
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URI: http://hdl.handle.net/10818/33167Visitar enlace: https://www.ajol.info//index.p ...
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ISSN: 1596-5996
DOI: 10.4314/tjpr.v15i6.19
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Pastrana Camacho, Natalia; Suárez, Zulma; Acosta González, Alejandro; Arango, Carolina; Haltli, Brad Arnold; Correa, Hebelin; Kerr, Russel; Duque, Carmenza; Díaz Barrera, Luis EduardoDate
2016Abstract
Purpose: To Isolate and characterize Actinobacteria with antimicrobial activity from Guaviare River
(Colombia).
Methods: Water and sediment samples were collected from Guaviare River. Direct plating, heat and
CaCO3 methods were used to isolate Actinobacteria. Six bacterial strains were tested using T-Streak
method: Escherichia coli ATCC 23724, Staphylococus aureus ATCC 25923, Acinetobacter baumannii
ATCC 19606, Bacillus subtilis ATCC 21556, Klebsiella pneumoniae ATCC 700603, Chromobacterium
violaceum ATCC 31532. Strains of Fusarium sp. H24, Trichoderma harzianum H5 and Colletotrichum
gloeosporioides were tested using Kirby-Bauer method. Isolates with high antimicrobial activity were
selected for further taxonomic identification.
Results: A total of 374 actinobacteria isolates were obtained. Seven isolates exhibited high
antimicrobial activity (p < 0.05) and were confirmed as members of Streptomycetaceae family. Of these,
three isolates showed differential phenotypic and genotypic profiles, indicating that they may represent
new species.
Conclusions: To date, this is the first study of this type in Colombian Orinoquia and indicates that this
promising source of Actinobacteria from aquatic sediments with the ability to produce antimicrobial
secondary metabolites.
Keywords
Ubication
Trop J Pharm Res, June 2016; 15(6): 1259
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- Facultad de Ingeniería [408]