@misc{10818/49620,
year = {2006},
url = {http://hdl.handle.net/10818/49620},
abstract = {To evaluate the role of tumor necrosis factor–α (TNF-α) gene as susceptibility marker for spondyloarthritis (SpA), two polymorphisms (−238 and −308 positions) were analyzed in 229 patients with SpA (113 with ankylosing spondylitis [AS], 92 with undifferentiated SpA [U-SpA], 24 with reactive arthritis), and 169 ethnically matched healthy control subjects. The HLA-B alleles were detected by PCR-SSP technique and the TNF-α polymorphism by PCR-RFLP. In comparison with healthy control subjects, the frequencies of TNF-238 in SpA were similar. In contrast, the analysis of −308 polymorphism showed increased frequencies of the T2(A) allele in the whole SpA group (p < 0.05, pC = NS, OR = 1.83) as well as the T2(A) allele (pC < 0.05, OR = 2.4) and T1T2(AG) genotype (p < 0.05, pC = NS, OR = 2.25) in U-SpA patients. Comparison of B27-negative patients and healthy control subjects yielded similar results. There was no significant correlation between TNF genotypes and clinical data. The present study demonstrates that TNF-α −308 polymorphism appears to be associated with the genetic susceptibility U-SpA. The association seems independent of the susceptibility conferred by the HLA-B27 in this group of patients.},
abstract = {Para evaluar el papel del gen del factor de necrosis tumoral alfa (TNF-alfa) como marcador de susceptibilidad para la espondiloartritis (SpA), se analizaron dos polimorfismos (posiciones -238 y -308) en 229 pacientes con SpA (113 con espondilitis anquilosante [EA] , 92 con SpA indiferenciada [U-SpA], 24 con artritis reactiva) y 169 sujetos de control sanos agrupados étnicamente. Los alelos HLA-B fueron detectados por la técnica PCR-SSP y el polimorfismo TNF-alfa por PCR-RFLP. En comparación con sujetos control sanos, las frecuencias de TNF-238 en SpA fueron similares. Por el contrario, el análisis del polimorfismo -308 mostró frecuencias aumentadas del alelo T2(A) en todo el grupo SpA (p < 0,05, pC = NS, OR = 1,83) así como del alelo T2(A) (pC < 0,05 , OR = 2,4) y genotipo T1T2(AG) (p < 0,05, pC = NS, OR = 2,25) en pacientes con U-SpA. La comparación de pacientes B27 negativos y sujetos de control sanos arrojó resultados similares. No hubo una correlación significativa entre los genotipos de TNF y los datos clínicos. El presente estudio demuestra que el polimorfismo TNF-alfa-308 parece estar asociado con la susceptibilidad genética U-SpA. La asociación parece independiente de la susceptibilidad conferida por el HLA-B27 en este grupo de pacientes.},
publisher = {Human Immunology},
title = {Tumor Necrosis Factor-α Promoter Polymorphisms in Mexican Patients With Spondyloarthritis},
title = {Polimorfismos del promotor del factor de necrosis tumoral alfa en pacientes mexicanos con espondiloartritis},
doi = {10.1016/j.humimm.2006.07.009},
author = {Vargas Alarcón, Gilberto and Casasola Vargas,  Julio and Rodríguez Pérez,  José Manuel and Pérez-Hernández, Nonanzit and Londoño, Juan and Pacheco Tena, César and Cardiel, Mario H. and Granados,  Julio and Burgos Vargas, Rubén},
}