%0 Generic
%A Morales Gonzalez, Maria
%A Martinez, Boghos Stepanian
%A Ramirez Rodriguez, Laura
%A Gómez, JEC
%A Diaz, LE
%8 2019-03-16
%@ 1596-5996
%U http://hdl.handle.net/10818/43796
%X Purpose: To optimize the L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia.

Methods: Actinobacterial strains were evaluated for their L-asparaginase activity using phenol red plates and Nessler’s assays. Strains with L-asparaginase activity were identified based on 16S ribosomal rRNA sequencing, and a central composite design was used to study nutritional and growth factors that could improve L-asparaginase activity. L-asparaginase protein was detected using western blotting and the cytotoxicity of L-asparaginase preparations was evaluated against MDA-MB231 and L929 cell lines.

Results: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces panaciradicis were cultured in medium with lactose as a carbon source and a combination of asparagine and malt extract as nitrogen sources. These strains showed L-asparaginase activities of 29.4, 114.06, and 34.08 U/mg, respectively, and half-maximal inhibitory concentration (IC50) values of 25.61 ± 2.15, 8.18 ± 1.61, and 165.29 ± 1.06 ppm, respectively, against MDA-MB 231 cells. Western blotting analysis revealed the presence of an L-asparaginase monomer with a molecular weight of 37 kDa.

Conclusion: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces Panaciradicis produce L-asparaginases with low L-glutaminase activity and promising cytotoxic activity and thus may be useful for the management of acute lymphoblastic leukemia.
%I Tropical Journal of Pharmaceutical Research
%K L-asparaginase
%K Streptomyces
%K Kitasatospora
%K Optimization
%K Cytotoxicity
%T Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia
%R 10.4314/tjpr.v17i11.13
%~ Intellectum