%0 Generic
%A Arango, Carolina
%A Acosta Gonzalez, Alejandro
%A Parra Giraldo, Claudia M.
%A Sánchez Quitian, Zilpa A.
%A Kerr, Russell
%A Díaz Barrera, Luis Eduardo
%8 2018-05-31
%U http://hdl.handle.net/10818/42362
%X Introduction:
New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

Material and Methods:
An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.

Results:
Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.

Conclusion:
The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.
%I The Open Microbiology Journal
%K Actinobacteria
%K Antimicrobial
%K Bioprospecting
%K MALDI-TOF MS
%K Physicochemical pretreatments
%K Taxonomic dereplication
%T Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates
%R 10.2174/1874285801812010181
%~ Intellectum