%0 Thesis
%A Bustos Cruz, Rosa Helena
%A Zapata, Carlos
%A Esteban Mancera, Efraín Arsenio
%A García Casallas, Julio César
%A Jáuregui, Edwin
%A Jaimes Fernández, Diego Alejandro
%8 2018-02-21
%U http://hdl.handle.net/10818/34885
%X This study describes the development of a immunosensory label-free quantification methodology based on surface plasmon resonance (SPR) and its applicability in measuring/evaluating therapeutic drug monitoring (TDM) of anti-TNF-alpha monoclonal antibody (adalimumab) in rheumatoid arthritis (RA) patients. The experimental parameters evaluated in this study were immobilising ligands by pre-concentration assays, sensor surface regeneration, ascertaining the method’s sensitivity and correlating the results from quantifying plasma samples by ELISA immunoassay. The results showed that SPR immunosensory detection provided a precise, sensitive strategy, along with real-time determination, for quantifying adalimumab, having great potential for clinical routine regarding TDM
%I Universidad de La Sabana
%K Adalimumab
%K Medicamentos -- Efectos fisiológicos
%T Label-free quantification of anti-TNF-alpha in patients treated with adalimumab using an optical biosensor
%~ Intellectum