%0 Journal Article
%A Vargas, Diana
%A Shimokawa, Noriaki
%A Kaneko, Ryosuke
%A Rosales, Wendy
%A Parra, Adriana
%A Castellanos, Ángela
%A Koibuchi, Noriyuki
%A Lizcano Losada, Fernando
%8 2016-02
%U http://hdl.handle.net/10818/33064
%X Increasing thermogenesis in white adipose tissues can be used to treat individuals at high risk
for obesity and cardiovascular disease. The objective of this study was to determine the
function of EP300-interacting inhibitor of differentiation (EID1), an inhibitor of muscle
differentiation, in the induction of beige adipocytes from adipose mesenchymal stem cells
(ADMSCs). Subcutaneous adipose tissue was obtained from healthy women undergoing
abdominoplasty. ADMSCs were isolated in vitro, grown, and transfected with EID1 or EID1
siRNA, and differentiation was induced after 48 h by administering rosiglitazone. The effects
of EID1 expression under the control of the aP2 promoter (aP2-EID1) were also evaluated in
mature adipocytes that were differentiated from ADMSCs. Transfection of EID1 into ADMSCs
reduced triglyceride accumulation while increasing levels of thermogenic proteins, such as
PGC1a, TFAM, and mitochondrial uncoupling protein 1 (UCP1), all of which are markers of
energy expenditure and mitochondrial activity. Furthermore, increased expression of the
beige phenotype markers CITED1 and CD137 was observed. Transfection of aP2-EID1
transfection induced the conversion of mature white adipocytes to beige adipocytes, as
evidenced by increased expression of PGC1a, UCP1, TFAM, and CITED1. These results indicate
that EID1 can modulate ADMSCs, inducing a brown/beige lineage. EID1 may also activate
beiging in white adipocytes obtained from subcutaneous human adipose tissue.
%I Journal of Molecular Endocrinology
%K Retinoblastoma
%K ADMSCs
%K PPARg
%K EID1
%K Beige fat
%T Regulation of human subcutaneous adipocyte differentiation by EID1
%R 10.1530/JME-15-0148
%~ Intellectum