%0 Generic
%A Conde Martínez, Natalia
%A Acosta González, Alejandro
%A Díaz Barrera, Luis Eduardo
%A Tello Camacho, Edisson
%8 2017-12-08
%@ 1471-2180
%U http://hdl.handle.net/10818/33036
%X Water evaporation in solar salterns creates salinity gradients that promote the adaptation of microbial species to different salinities. This competitive habitat challenges the metabolic capabilities of microorganisms and promotes alterations in their production of secondary metabolites. Thus, solar salterns are a potentially important source of new natural products. In Colombia, the most important and representative solar saltern is located in Manaure (La Guajira) in the north of Colombia. The aim of this study was to develop an alternative screening strategy to select halophilic bacteria as producers of bioactive compounds from mixed microbial cultures rather than individual environmental isolates. Brine and sediment samples from different ponds (across a salinity gradient) were inoculated in seven different culture media to grow bacteria and archaea, allowing for a total of 40 different mixed cultures. An organic extract from each mixed culture was obtained and tested against multidrug resistant pathogens, including Klebsiella pneumoniae, vancomycin-resistant Enterococcus faecium, methicillin-resistant Staphylococcus aureus and Bacillus subtilis. In addition, the extracts were tested against two human cancer cell lines, cervical adenocarcinoma (SiHa) and lung carcinoma (A-549).
%I BMC Microbiology
%K Mixed cultures
%K Halophiles
%K Cytotoxic activity
%K Antibacterial activity
%K Solar salterns
%T Use of a mixed culture strategy to isolate halophilic bacteria with antibacterial and cytotoxic activity from the Manaure solar saltern in Colombia
%R 10.1186/s12866-017-1136-x
%~ Intellectum