@misc{10818/42362,
year = {2018},
month = {5},
url = {http://hdl.handle.net/10818/42362},
abstract = {Introduction:
New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

Material and Methods:
An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.

Results:
Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.

Conclusion:
The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.},
publisher = {The Open Microbiology Journal},
keywords = {Actinobacteria},
keywords = {Antimicrobial},
keywords = {Bioprospecting},
keywords = {MALDI-TOF MS},
keywords = {Physicochemical pretreatments},
keywords = {Taxonomic dereplication},
title = {Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates},
doi = {10.2174/1874285801812010181},
author = {Arango, Carolina and Acosta Gonzalez, Alejandro and Parra Giraldo, Claudia M. and Sánchez Quitian, Zilpa A. and Kerr, Russell and Díaz Barrera, Luis Eduardo},
}